Thp1 monocyte
WebEvidence for a CD14- and serum-independent pathway in the induction of endotoxin-tolerance in human monocytes and THP-1 monocytic cells. Tumor necrosis factor alpha Stimulation Secretion MAPK/ERK pathway Biology Internal medicine Endocrinology Molecular biology Antibody Kinase Chemistry THP1 cell line TLR4 CD14 … WebNov 18, 2015 · Previous analyses have reported that the human monocytic cell line THP1 can be differentiated into cells with macrophage-like characteristics by phorbol 12 …
Thp1 monocyte
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WebJul 9, 2015 · THP1 (ATCC ® TIB-202™) human monocyte cell lines were cultured in Roswell Park Memorial Institute medium 1640 containing 10% (v / v) fetal bovine serum, 0.05 mM 2-mercaptoethanol, 100 U/mL penicillin, 100 μg/mL streptomycin, and 0.5 μg/mL amphotericin B (Wako Pure Chemicals, Osaka, Japan) at 37 °C, 5% CO 2, and in a humidified cell culture … Web- Work with aseptic techniques and maintain our cellular line of THP1 monocytes. - Used trypan blue dye for viable cell counting with the Neubauer chamber. - Sample preparation with Indoxyl Sulfate toxin and LPS to measure different processes in our cells, such as Reactive Oxidative Stress, Apoptosis and phenotype in different timings.
WebMar 30, 2024 · THP1 (human monocyte cell line), A2780 (human ovarian cancer cell line), and OVCAR8 (human ovarian cancer cell line) cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco, 11875176). All media were supplemented with 10% fetal bovine serum (FBS) ... WebEndothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion Jakub Rohlena1†, Oscar L. Volger2, Jaap D. van Buul3, Liesbeth H.P. Hekking4, Janine M. van Gils3, Peter I. Bonta1, Ruud D. Fontijn2, Jan Andries Post4, Peter L. Hordijk3, and Anton J.G. Horrevoets2* 1Department of Medical Biochemistry, Academic …
WebTHP-1. TIB-202 ™. THP-1 is a monocyte isolated from peripheral blood from an acute monocytic leukemia patient. This cell line can be used in immune system disorder research, immunology research, and toxicology research. Note: These are suspension cells and … The Credible Conversations blog shares the science and innovation from inside … Certain materials in the collection may require one or more permits to be filled … ATCC is a nonprofit organization that collects, stores, and distributes standard … WebTHP1 is a human AML cell line that resembles human monocytes, which was used as a model to measure platelet-monocyte aggregation. The blood samples were stimulated by …
WebDec 1, 2010 · The aim of our study was to identify and validate reliable reference genes for the THP-1 cell culture model of monocyte maturation. For this we have preselected 21 …
WebInvivoGen thp1 dual tm monocyte cell line Thp1 Dual Tm Monocyte Cell Line, supplied by InvivoGen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed … crewe archivesWebDec 25, 2024 · Here, we investigated if surface modification of AgNP by liposomal encapsulation suppresses AgNP-mediated inflammatory responses in THP1 monocytes … crewe ao warehouseWebApr 13, 2024 · HIGHLIGHTS. who: Maria Rynikova and colleagues from the Department of Animal Physiology, Faculty of Science, Pavol Jozef Safarik University in have published the Article: Transcriptomic Analysis of Macrophage Polarization Protocols: Vitamin D3 or IL-4 and IL-13 Do Not Polarize THP-1 Monocytes into Reliable M2 Macrophages, in the … buddhist library sydneyWebMonocytes are differentiated into macrophages. In this study, mitochondrial DNA copy number (mtDNAcn) levels and downstream events such as the expression of respiratory chain mRNAs were investigated during the phorbol 12-myristate 13-acetate (PMA)-induced differentiation of monocytes. buddhist life is sufferingWebAtrial natriuretic peptide (ANP), a cardiovascular hormone, elicits different biological actions in the immune system. Aim of the present study was to investigate in THP-1 monocytes the ANP effect on hydrogen peroxide (H2O2)-induced Reactive Oxygen Species (ROS), cell proliferation and migration. buddhist life cycleWebTHP-1 human monocyte (ATCC® TIB-202™) cell line was obtained from American Type Culture Collection. The cells were grown in RPMI 1640 medium supplemented with 2 mM L-glutamine, 10% fetal bovine serum and 1% penicillin-streptomycin and 2-mercaptoethanol to a final concentration of 0.05 mM at a ... buddhist lids craftsWebDec 1, 2024 · THP-1 cells are often used as model to study macrophage function, however they may act differently from primary human monocyte derived macrophages (MDMs). … crewe area rental property below £680