Rab7 protein purchase
WebThe cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab137029, 0.1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor ® 488 goat anti-rabbit IgG (H&L) ( ab150081 ) at 1/2000 dilution for 30 min at 22°C. WebOct 16, 2014 · In vitro GTP gamma S/GDP Protein Loading for positive and negative controls Note: In vivo stimulation of cells will activate approximately 10% of the available Rab7, whereas in vitro GTP gamma S protein loading will activate nearly 90% of Rab7. 1. Aliquot 0.5 mL of each cell extract to two microfuge tubes (or use 1 μg of purified Rab7 protein). 2.
Rab7 protein purchase
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WebNov 3, 2024 · Fig. 9: Rab7 activation by the MCC GEF controls NPC1-dependent lysosomal cholesterol export. a–d Lysosomal cholesterol export is abolished in NPC1 -, C18orf8 -, … WebOct 19, 2024 · Rab7 is a small G protein in the Rab family. There are two types of Rab7 in mammals, Rab7a and Rab7b, which are 50% homologous and act at different stages of membrane transport 7.
WebCatalog FieldsAntigen: RAb7/CG5915 proteinAvailable to For-Profits: YesAntigen Species: DrosophilaHybridoma Cells Available (Non-Profit): YesIsotype: MIgG1, kappa light … WebJan 1, 2024 · Triple-negative breast cancer (TNBC) is a heterogeneous disease enriched for mutations in PTEN and dysregulation of innate immune signaling. Here, we demonstrate that Rab7, a recently identified substrate of PTEN phosphatase activity, is also a substrate of the innate immune signaling kinases TANK-binding kinase 1 (TBK1)/IκB kinase ε (IKKε) on the …
WebRab7 recruits new proteins to maturing phagosomes. Only few Rab7 effectors have been characterized which include Oxysterol-binding protein related protein 1 (ORPL1) and Rab7 … WebRAB7A Antibody (PA5-78238) in ICC/IF. Immunofluorescence analysis of Ras-related protein Rab-7a was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature.
WebRab7 is a late endosome-/lysosome-associated small GTPase, perhaps the only lysosomal Rab protein identified to date. Rab7 plays critical roles in the endocytic processes. …
WebSep 22, 2024 · The switch I/II and interswitch regions of the GTP-loaded Rab7 form the binding interfaces, which correlates with the GTP-dependent interaction of PDZD8 and Rab7. Analysis of the protein ... subway summarecon bekasiWebRAB7 Antibodies. Antibodies that detect RAB7 can be used in several scientific applications, including Immunohistochemistry, Western Blot, Immunocytochemistry, Flow Cytometry … subway sullivan moWebPurchase online Abcam homepage. Research Products. By product type Primary antibodies Secondary ... Ras related protein Rab7 antibody; Ras related protein Rab7a antibody; Ras-related protein Rab-7a antibody; see all. Images. AP Rabbit monoclonal [EPR7589] to RAB7 (ab308807) Protocols. subway summerville ga