Poly-ubiquitinated
WebApr 7, 2011 · CAIR-1/BAG-3 abrogates heat shock protein-70 chaperone complex-mediated protein degradation: accumulation of poly-ubiquitinated Hsp90 client proteins. J Biol Chem 2003; 278 : 28490–28500. WebJun 2, 2015 · The nanomolar affinity of TUBEs for poly-ubiquitinated proteins allows the efficient isolation and identification of ubiquitinated proteins from cells, tissues and organs. To learn more about TUBEs, their benefits and potential applications TUBEs (WB, pull downs, histochemistry, fluorescence microscopy), take a look at TUBEs – efficiently detect & …
Poly-ubiquitinated
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WebThis powerful platform is an improvement on the most common method for investigating protein ubiquitination: immunoprecipitation and Western blot analysis. However, the substrate antibody often interacts differently with the poly-ubiquitinated forms of the substrate in the immuno-blotting step. WebSubsequently, most of these ubiquitinated (generally poly-ubiquitinated) proteins are recognized and turned over by the proteasome, which is a large multi-subunit complex of proteases and regulatory proteins (for more details about the UPS and proteasome structure, see References [2,5,6,7]).
WebMultiDsk-Ubiquitin-binding protein reagent This MultiDsk-Ubiquitin-binding protein reagent is validated for use in Affinity Precipitation Assays for the pull-down of mono- and poly … WebMar 27, 2009 · The yeast G protein alpha subunit Gpa1 represents a rare example of a protein that undergoes both mono- and poly-ubiquitination. Whereas mono-ubiquitinated Gpa1 is targeted to the vacuole, poly-ubiquitinated Gpa1 is directed instead to the proteasome. Here we investigate the structural requirements for mono- and poly …
Web2 days ago · SUMO-modified PML/RARα is targeted by the E3 ubiquitin ligase RNF4, which has highly selective activity for ubiquitylation and degradation. 29 Arsenic trioxide, the first-line drug used to treat APL with a good prognosis, induces SUMOylation of PML/RARα at Lys160, allowing RNF4 to target the poly SUMO-2 chain and thus triggering the … WebThe proteasome is a ubiquitous multi-subunit enzyme complex that plays a critical role in the degradation of poly-ubiquitinated proteins to produce small peptides. 6 Ubiquitin-independent proteasomal degradation has also been reported, provided that the target contains intrinsically disorganized regions. 7 Many subunits of the proteasome are ...
WebMar 25, 2016 · Ubiquitin can be ubiquitinated on seven lysine (Lys) residues or on the N-terminus, ... SUMOylation of (poly)ubiquitin is conceptually similar to polyubiquitin chain formation.
WebIt is important to distinguish between poly-ubiquitination and multi-mono-ubiquitination because these different types of ubiquitination lead to different functions of the substrate … hideaway ccrWebMar 21, 2024 · The RGS4 down-regulation appears completely blocked by MG-132 pretreatment or by the specific proteasome inhibitor lactacystin and, accordingly, the protein remains strongly poly-ubiquitinated. In other words, authors suggest that DAMGO or DPDPE treatment promote the RGS4 poly-ubiquitination which normally acts as a signal … hideaway cave creek facebookWebJan 28, 2024 · Lys 63 ubiquitin chains are conjugated to Lys13-15 mono-ubiquitinated H2A/H2AX and the resulting poly-ubiquitinated histones facilitate the DNA damage response (DDR) . 53BP1 is a chromatin-associated factor that promotes DSB repair by nonhomologous end joining. 53BP1 binds mono-nucleosomes including demethylated … hideaway cell phoneWebRat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ELISA Kit. Detection Target: ubiquitin carboxyl-terminal esterase L1 (ubiquitin ... Detection Range: 0.78 ng/mL-50 … hideaway cell phone carrierWebMay 4, 2014 · The poly-ubiquitinated α-synuclein products obtained were used to investigate the effect of the Ub chain length on the proteosomal degradation of the … hideaway ceiling tv mountWebOct 26, 2016 · 1- Check what is the half life of your overexpressed protein-X. That always affect the overall ubiquitination levels in the lyaste at a given time upon MG132 treatment. … hideaway chair coffee tableWebMay 1, 2024 · However, PCNA is also ubiquitinated during normal S-phase progression. ... mono-ubiquitination is the prevalent form of modified PCNA, although poly-ubiquitination … hideaway chair