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Phm6 vector

WebbOpen reading frame of c-myc was cloned into pHM6 vector. These PKM2, PKM2 mutants, and PKM1 expression vector carry mutations at the PKM2 siRNA targeting site, therefore there are resistant to siRNA knockdown. For bicistronic vectors, β-actin ORF was synthesized and inserted into pEGFP-N1 vector with HA tag in N-terminal of . Webb21 aug. 2008 · Identification of TUSC4/NPRL2 as a PDK1-interacting protein. (a) After cloning the full-length cDNA of each PDK1 binding protein (BP), we transfected a pc5FLAG vector encoding none (mock, lanes 1 and 2), full-length candidate proteins (BP1-BP8 and TUSC4, lanes 3–11), or 14-3-3θ (lane 12), together with a pHM6 vector encoding none (–, …

Phm6 Vector Roche Bioz

Webba pc5FLAG vector was generated by polymerase chain reaction (PCR) with an I.M.A.G.E clone (clone ID 6169151; Open Biosystems, Huntsville, AL, USA) as the template. The p60 c-src cDNA in a pHM6 vector was generated by PCR with an I.M.A.G.E clone (clone ID 4548399; Open Biosystems) as the template. Deletion mutants of TUSC4 were … Webb15 juni 2003 · Oligonucleotides carrying Bsu15I and Psp5II restriction sites were introduced in SacII-NotI sites of pHM6 vector. The cDNA encoding the N-terminal part of PTPL1 was cloned by PCR using pSV7d-PTPL1 (gift from C. H. Heldin, Ludwig Institute for Cancer Research, Uppsala, Sweden) as a template in Sac II- Bsu 15I sites of modified pHM6 … five dollar box from taco bell https://boxtoboxradio.com

PHM6 SGD

Webb1 aug. 2008 · The HA-tagged protein was obtained from a pHM6 vector encoding no protein (Mock). HA-tagged Identification of the domains responsible for interactions between Freud-1/Aki1 and PDK1. (A) The... WebbThe pES6, pHM4, pHM5 and pHM6 vectors were derived from the pES4 plasmid containing bgl1, celA-celB, celA and celB synthetic genes, respectively. All of the genes were flanked by a synthetic... WebbPuseamp Vector, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 80/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > Upstate Biotechnology Inc > puseamp vector. puseamp vector (Upstate Biotechnology Inc) About; five dollar box at taco bell

Production and characterization of fungal - SpringerLink

Category:TUSC4/NPRL2, a novel PDK1‐interacting protein, inhibits PDK1 …

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Phm6 vector

Freud-1/Aki1, a novel PDK1-interacting protein, functions as a …

WebbPlasmid pHM6-alphasynuclein-WT from Dr. David Rubinsztein's lab contains the insert SNCA and is published in Biochem J. 2000 Mar 15;346 Pt 3:577-81. This plasmid is … WebbpHM6 87228 ™ Organism Yersinia enterocolitica subsp. enterocolitica (Schleifstein and Coleman) Frederiksen Clone type Clone Applications Molecular biology Product format …

Phm6 vector

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http://www.protocol-online.org/biology-forums/posts/5295.html WebbThe product is provided 'AS IS' and the viability of ATCC ® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the …

http://www.protocol-online.org/biology-forums/posts/5295.html WebbThe HA-AS160(1–532) construct was obtained by PCR amplification with primers containing KpnI and EcoRI restriction sites and cloned into the pHM6 vector (Roche Applied Science).

Webb(a) Immunoblot analysis of MCF7 cells, which were stably transfected with HA-Aurora-A and pHM6 vector, with anti-HA and anti-ERα and anti-actin antibodies. (b–d) Aurora-A- and pHM6... Webb17 juni 2005 · Human wild-type ILK cDNA (WT-ILK) in a pFLAG-CMV-2 vector and a pHM6 vector were established in our laboratory. Several ILK fragments that encompassed residues 1–406 (ΔC407- ILK ) and 1–375 (ΔC376- ILK ) were generated by converting appropriate codons in WT- ILK cDNA to stop codons using a QuickChange site-directed …

WebbBasic Vector Information; Vector Name: pHM6: Antibiotic Resistance: Ampicillin Length: 5450 bp: Type: Mammalian Expression Vectors Selection Marker: …

WebbMammalian Expression Vectors— pHM6 was used to express molecules tagged at the N terminus with hemagglutinin (HA) in mammalian cells (driven by a cytomegalovirus immediate early promoter). Construction of pHM6-Cezanne 1–858 and pHM6-Cezanne 1–443 has been described previously ( 11 ). can intersex catholics get marriedWebb16 juni 2014 · OK cells were transiently transfected with pHM6-DNPEP, pHM6-(H94F) DNPEP, or pHM6 empty vector (control) using Lipofectamine 2000 (Invitrogen). Forty-eight hours posttransfection, confluent OK cell monolayers were washed three times in ice-cold PBS, and then biotinylated with EZ-Link NHS-SS- Biotin (Pierce) at 4°C with gentle agitation. can interpret those scary healthWebbBoehringer Mannheim phm6 vector Phm6 Vector, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO … five dollar eshop gift cardWebbcloned into blunted HindIII(5=)-BamHI(3=) sites of expression vector pHM6 (Invitrogen, Carlsbad, CA), while the 988 bp rat mas PCR fragment was cloned into HindIII (5=)-EcoRI (3=) sites of pHM6 after digestion with HindIII and EcoRI. Chemicals. Mas agonist (AR234960) and inverse agonists (AR244555 and AR305352) were synthesized at Arena … can intersex impregnate themselvesWebbExpression Plasmid Vector Phm6, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … can intersecting lines be coplanarWebb28 juli 2008 · Freud-1/Aki1 in the pCRII vector was digested with EcoRI and cloned into the EcoRI site of a pFLAG-CMV-2 (Sigma) or pHM6 vector (Roche Diagnostics, Indianapolis, IN). The Freud-1/Aki1 mutants were generated by PCR with pFLAG-CMV-2-WT-Freud-1/Aki1 as the template, using a QuickChange mutagenesis kit (Stratagene). five dollar fine for whiningWebbVector Type Resistance Marker Bacterial Resistance Source Sequence Available; pHM6: Mammalian Expression: Neomycin: Ampicillin: Roche five dollar fee in spanish