WebJan 4, 2024 · Understanding the context-specific role of gene function is a key objective of modern biology. To this end, we generated a resource for inducible cell-type specific trans-activation based on the well-established combination of the chimeric GR-LhG4 transcription factor and the synthetic pOp promoter. Harnessing the flexibility of the GreenGate … WebJan 20, 2024 · Here, we adapted the GreenGate cloning vectors to create two destination vectors showing strong marking of cell membranes in either the whole root or specifically in the lateral roots. This system can also be used in both embryos and whole seedlings. As proof of concept, we follow both gene expression and anatomy in Arabidopsis …
GreenGate - TypeIIS RE Cloning System for Plant Transgenesis
WebOct 12, 2024 · The hip1 coding sequence devoid of BsaI restriction sites for GreenGate cloning was synthesized by Biocat (Germany). For recombinant protein expression, the hip1 sequence was amplified without SP and cloned into the pET28a (+) expression vector (Addgene, USA), eventually having a His-tag at the N-terminus. Sequences for cysteine … Webgreen gate cloning 중인데 cloning이 처음입니다 mod c인 cds를 만들던 중 프라이머 Forward에... birth certificate apostille philippines
National Center for Biotechnology Information
Weband versatile cloning system for the generation of plant transfor-mation vectors, which we named GreenGate. The GreenGate system allows rapid and efficient assembly of six … WebThe characteristic of GreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors fused with different tags. On the basis of the … WebDec 19, 2013 · A) The GreenGate cloning system uses six different types of pUC19 based entry vectors into which the individual elements are inserted and a pGreen-IIS based destination vector. Magenta scissors represent BsaI recognition sites. In each GreenGate reaction, six modules are ligated between the left border (LB) and the right border (RB) … daniel chan understand your everything