Graphene based gene transfection
WebGene therapy has been found to hold tremendous potential for the treatment of cancers beyond conventional methods. 30–32 Nonviral vector provides safer methods without the potential side effects of viral transfection, such as immunogenicity and carcinogenesis. 33 GO has been considered an ideal vector for gene delivery. 34,35 In this study ... WebVisualizing The Localization Of Transfection Complexes During Graphene Nanoparticle-Based Transfection Journal of Materials Chemistry B …
Graphene based gene transfection
Did you know?
WebJan 10, 2014 · Successful gene therapy requires efficient and safe gene vectors that protect DNA from nuclease degradation as well as facilitate DNA uptake with high transfection efficiency [139], [140]. Graphene has been explored for applications in gene delivery, gene–drug co-delivery and protein delivery with good supportive literature available. WebAug 18, 2024 · Deoxyribonucleic acid (DNA) vaccines are a promising cancer immunotherapy approach. However, effective delivery of DNA to antigen-presenting cells (e.g., dendritic cells (DCs)) for the induction of an adaptive immune response is limited. Conventional DNA delivery via intramuscular, intradermal, and subcutaneous injection by …
WebDec 7, 2024 · Graphene has been widely studied for biomedical use since its first appearance in 2004 [3], partly owing to its high Young's modulus, high fracture strength, high electrical conductivity, and excellent optical performance. Graphene also possesses a large specific surface area for adsorption of bioactive compounds and has high optical … WebJun 1, 2014 · Abstract. Graphene and carbon nanotube nanocomposite (GCN) was synthesised and applied in gene transfection of pIRES plasmid conjugated with green fluorescent protein (GFP) in NIH-3T3 and NG97 cell lines. The tips of the multi-walled carbon nanotubes (MWCNTs) were exfoliated by oxygen plasma etching, which is also …
WebJul 30, 2024 · Methods: Graphene oxide/APTES was modified by spermine (GOAS) and characterized by FT-IR, DLS, SEM and AFM techniques. Then pEGFP-p53 was loaded on GOAS, transfected into cells and evaluated by fluorescent microscopy and gene expression techniques. Results: FT-IR data approved the GOAS sheet formation. Ninety percent of … WebJun 18, 2024 · Abstract. Functionalized graphene oxide (GO) nanoparticles are being increasingly employed for designing modern drug delivery systems because of their high degree of functionalization, high surface area with …
WebOct 13, 2014 · Graphene based biosensors make use of two strategies-1) probe molecule on graphene interacts with the analyte (FRET based ... Zhang S, Liu Z (2011) Graphene based gene transfection. Nanoscale 3: 1252-1257. Zhou L, Wang W, Tang J, Zhou JH, Jiang HJ, et al. (2011) Graphene oxide noncovalent photosensitizer and its anticancer …
WebJan 6, 2024 · Graphene oxide for transfection. ThermoFisher Scientific describes transfection as the process of artificially introducing nucleic acids – DNA or RNA – into cells, utilising means other than viral infection. A paper published in Nanoscale 2011 titled ‘Graphene based gene transfection’ showed that graphene oxide (“GO”) bound with ... greatland payrollWebJan 23, 2024 · Recently graphene has been used in biomedical applications including DNA sequencing 10, biosensor development 11, and graphene-enhanced cell differentiation … greatland pbt 4346WebMany researchers have tried to transfect genes using carbon-based materials such as graphene oxides (GOs) and graphene quantum dots (GQDs) [29,30,31,32]. ... Prior to in vitro gene transfection with NGQDs, we verified the cytotoxicity of the NGQDs via a CCK-8 assay kit. For the CCK-8 assay, various concentrations of NGQDs from 1 μg/mL to 1000 ... floe boat lift 12 volt winchWebJan 15, 2024 · Graphene and graphene-based nanomaterials such as graphene oxide (GO), reduced graphene oxide (rGO) and graphene quantum dots (GQDs) ... Feng, S. Zhang and Z. Liu, Graphene based gene transfection, Nanoscale 3 (2011) 1252–1257. floe boat lift winchWebGraphene as a star in materials research has been attracting tremendous attentions in the past few years in various fields including biomedicine. In this work, for the first time we … greatland payroll softwareWebWe conclude that modification of graphene-based nanoparticles with PEG and especially PEI provide better colloidal stability in cell medium, resulting in more uniform transfection and overall increased efficiency. ... Liu, Z. Graphene based gene transfection. Nanoscale 2011, 3, 1252–1257. [Google Scholar] Liu, Z.; Robinson, J.T.; Sun, X.; Dai ... floe bottleWebJun 18, 2024 · Graphene-oxide-based nanocarriers have shown great potential in gene transfection and could be used in breast cancer treatments by overcoming the limitations and side effects of chemotherapy. This carbon-based material allows a facile surface modification to enhance its biological application and gene delivery efficiency. floeckscountry.com