Fluorochrome spectra
WebApr 14, 2024 · The Excitation and Emission spectra. Fluorochromes absorb and emit light at varying intensities across the spectrum, with each fluorochrome having distinct absorption and emission spectra. These spectra indicate that a specific fluorochrome can absorb adjacent wavelengths, but its maximum absorption occurs in the peak … WebPE-Cy7 is a tandem fluorochrome composed of R-phycoerythrin (PE), which is excited by 488-nm light and serves as an energy donor, coupled to the cyanine dye Cy7, which acts as an energy acceptor and fluoresces maximally at 780 nm. PE-Cy7 tandem fluorochrome emission is collected in a detector for fluorescence wavelengths of 750 nm and higher.
Fluorochrome spectra
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WebFluorochromes have spectral characteristics that allow them to absorb light of certain wavelengths and then to emit light at longer wavelengths. Emission, a specific characteristic of the compound, is not limited to a fixed wavelength but rather constitutes a spectrum, with variable numbers of photons emitted at different wavelengths. WebFluoroFinder Spectra Viewer is an interactive platform that facilitates fluorescence experiment design. View and compare the spectral properties of more than 1,000 dyes …
WebFor fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. WebThe excitation spectrum of a given fluorochrome is determined in a similar manner by monitoring fluorescence emission at the wavelength of maximum intensity while the fluorophore is excited through a group of consecutive …
WebThe BD Spectrum Viewer is a tool that depicts the excitation and emission curves of fluorochromes common to flow cytometry. This tool can be used to determine … Web191 rows · Spectra Viewer. Add some data! Use the legend to add fluorochromes, filters sets and individual filters to the plot. All transmission and blocking (OD) data are actual, …
WebFluorescence SpectraViewer Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm.
WebFor fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). irish ferries fishguard to rosslare timetableWebCyan Fluorescent Protein (CFP) is a versatile biological marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. CFP can be excited by the 405 nm laser line and is optimally detected at 485 nm. irish ferries freight holyhead to dublinWebA fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain … irish ferries dublin to holyhead timetableWebThese spectra are generated by an instrument called a spectrofluorimeter, which is comprised of two spectrometers: an illuminating spectrometer and an analyzing spectrometer. First the dye sample is strongly illuminated … porsche taycan dealer near west covinaWebDriven by BD innovation, BV480 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 480-nm (e.g., a 525/50 bandpass filter). … porsche taycan dealsporsche taycan delivery times ukWebPerCP-Cy5.5 is optimized for use with a single argon ion laser emitting 488-nm light. Because of the broad absorption spectrum of the tandem fluorochrome, extra care must be taken when using dual-laser cytometers, which … irish ferries holidays to ireland