WebGibco™ HT Supplement is a liquid mixture of sodium hypoxanthine (10 mM) and thymidine (1.6 mM). HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular … WebCorning® HT (Hypoxanthine, Thymidine), Corning. Supplier: Corning. HT media is a 'rescue media' that provides pre-formed purines and pyrimidines to overcome the effects of residual intracellular aminopterin. Complete Certificate of Analysis available for each production lot, along with full formulation information. Produced under the highest ...
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WebSep 1, 2012 · In our previous study, we demonstrated that combinatorial addition of hypoxanthine (10 mg/L) and thymidine (2 mg/L) was able to stimulate initial cell growth and elevate volumetric concentration of antibody by 22% (Chen et al., Appl. Microbiol. Biotechnol., 93, 169–178, 2012). In this study, a systematic study was carried out to … WebJul 25, 2024 · 5) As Untransfected CHO-DG44 (DHFR negative) grows in media supplemented with Hypoxanthine and thymidine, Upon transfection in media lacking the suplementation of Hypoxanthine and thymidine … literature table for research
Hypoxanthine - an overview ScienceDirect Topics
WebJul 30, 2011 · The potential of hypoxanthine and thymidine (H&T) to promote growth of CHO cells and production of monoclonal antibody (mAb) was explored in this study. It was demonstrated that H&T stimulated the initial cell growth and enhanced volumetric production of anti-human CD20 mAb by 22%, mainly through the elevated integrated viable cell … WebMay 15, 1992 · With both dhfr expression plasmids, selection for dhfr+ transformants followed by single dilution cloning was sufficient to generate cell lines with a production level of up to 4.6 micrograms tPA/10 (6) cells.day. This approach is useful if gene amplification procedures are time-consuming and impracticable because of a large number of ... WebSep 21, 2024 · Transfected cells survive because they produce DHFR as well as upstream cloned GOI. Although MTX blocks DHFR, by challenging the cells with increasing … import inflation