WebJan 22, 2024 · The level of LDs was assayed using BODIPY 493/503 (#D3922, Thermo Fisher Scientific) according to the manufacturer's protocol. 2.5. Western blot. Western blot was performed as previously described [24]. In brief, proteins in the cell lysate or supernatants were resolved on 4%–12% Criterion XT Bis-Tris gels (#3450124, Bio-Rad, …
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WebMay 14, 2024 · Most recently, to understand the molecular etiology of thyroid-associated orbitopathy, our group utilized the 3D cell culture of human orbital fibroblasts, and found that hypoxia-inducible factor... WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 …
WebFeature papers are submitted upon individual invitation or recommendation by the scientific editors and must receive positive feedback from the reviewers. ... Alternatively, for BODIPY staining, 3D spheroids in 6 super-low-attachment well dishes were incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 1 h ... WebIECs were cultured in 12-well plates for 36 h, then washed twice with PBS and incubated with the 5 g mL −1 Bodipy 493/503 (D3922; Thermo Fisher Scientific, Waltham, MA, USA) for 30 min, followed by three PBS washes. A laser scanning confocal microscope (Leica DMI8) was used to visualize the fluorescence intensity in the IECs.
WebThermo Fisher Scientific is dedicated to improving the human condition through systems, consumables, and services for researchers. WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1 …
WebMay 14, 2024 · To analyze their lipid droplet formation for the 3D culture, the organoids were transferred to 6 super-low attachment well dishes, incubated in 0.2% BODIPY (# D3922, Thermo Fisher Scientific) in PBS for 1 hr, and then fixed in 4% paraformaldehyde (PFA) in PBS for 10 min at room temperature.
WebFeb 1, 2024 · We performed ORO and Bodipy 493/503 (D3922; Thermo Fisher Scientific, Waltham, MA, USA) staining based on the protocols described in our previous research [4, 13]. The ten fields were selected from each sample to qualify relative areas of LDs in ORO by Image J. We used the laser scanning confocal microscope (Leica Microsystems, … how to speed glitch in krunkerWebNov 18, 2024 · DFCP1 is an ER-associated protein with two PI (3)P-binding FYVE domains. In WT cells, myc-DFCP1 formed very small punctate structures ( Figure 1E) ( Axe et al., 2008; Li et al., 2024 ). In SKO cells, the myc-DFCP1 structures appeared highly irregular and significantly enlarged ( Figure 1, E and F). rcw for robbery 1Web货号: D3922 相关应用: Cellular Imaging 技术支持 客户服务 收藏夹 产品概述 推荐产品 文件 常见问题解答 引用和文献 BODIPY™ 493/503 具有非极性结构、吸收波长较长且发荧 … rcw for organized retail theftWebFeb 24, 2024 · Finally, BODIPY493/503 (#D3922, Thermo Fisher Scientific, USA) staining was performed to check the lipid droplet formation. ... USA) according to the manufacturer’s instruction. SYBR Green qPCR Master Mix (#K0251, Thermo Fisher Scientific, USA) was used for quantitative real-time PCR amplification with a CFX96 real-time PCR detection … rcw for racingWebSep 1, 2024 · Briefly, HepG2 cells were fixed with 4% paraformaldehyde for 10 min, then washed three times with phosphate buffer saline (PBS). After washing, cells were incubated with BODIPY 493/503 (D3922, Thermo Fisher Scientific, MA, USA) at room temperature for 1 h, followed by three washes with PBS. rcw for stolen license platesWebApr 20, 2024 · To assess the viability of the adipocytes on-chip, a live/dead-assay based on fluorescein diacetate (FDA; F1303, Thermo Fisher Scientific) and propidium iodide (PI; P3566, Thermo Fisher Scientific ... how to speed glitch in sword burst 2WebIn order to identify podocyte proteins that could perform as a similar or better biomarker for the presence of CPF (s), or are involved in the mechanism of action of CPF (s), we here followed a proteomics approach by analyzing proteomes of hPod exposed to presumably CPF-containing plasmas from patients with FSGS. rcw for possession of stolen property