B-tubulin molecular weight

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August undefined, 2024

WebTubulin, the subunit protein of microtubules, is a dimer that sediments at 6 S and has a molecular weight of 110,000. Using high resolution polyacrylamide gel electrophoresis, … WebMar 20, 2024 · It is a large protein; the molecular weight for human vinculin is 117 kDa with 1066 amino acids. It can be used as a loading control for high molecular weight proteins. In muscle tissues, a splice variant with an extra exon with the molecular weight of 150 kDa is also expressed. Nuclear proteins - nuclear loading controls Lamins

Loading Control Antibodies Cell Signaling Technology

WebIts molecular weight is approximately 42 kDa. References [ edit] ^ a b c GRCh38: Ensembl release 89: ENSG00000075624 - Ensembl, May 2024 ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000029580 - Ensembl, May 2024 ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine. Webtubulin, alpha 1b: Calculated molecular weight: 50 kDa: Observed molecular weight: 50-55 kDa: GenBank accession number: BC009314: Gene symbol: TUBA1B: Gene ID (NCBI) 10376: Conjugate: … braf inhibitor rash

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Webβ-tubulin belongs to the subfamily of tubulins, which are the major building blocks of microtubules. β-tubulin has a molecular weight of 55kDa. β-tubulin structure is … WebEach has a molecular weight of approximately 50 kDa. To form microtubules, the dimers of α- and β-tubulin bind to GTP and assemble onto the (+) ends of microtubules while in the GTP-bound state. The β-tubulin … Web[ 2] [ 1] TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000. [ 3] Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. braf inhibitor crc

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α/β-Tubulin Antibody Cell Signaling Technology

WebSelecting the right loading control antibody is determined by the molecular weight of the other targets being studied, subcellular fraction of the sample, and the cell and tissue types being evaluated. You need to trust that your loading control is … WebThough beta-actin has a molecular weight of 42 kDa, degradation by apoptosis will produce 38-41 kDa fragments on a Western blot. Proteintech’s monoclonal beta-actin antibody ( 60008-1-Ig) (Figure 1) recognizes all fragments, while 66009-1-Ig antibody (Figure 2) can only detect the full length of beta-actin. hackers cereal 90\\u0027sWebSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Protocol Specificity / Sensitivity The α/β-Tubulin Antibody detects endogenous … hackers chile

"WebA panel of docking scaffolds was developed for the known molecular targets of the anticancer agents, thieno[2,3-b]pyridines, in order to glean insight into their mechanism of action. The reported targets are the copper-trafficking antioxidant 1 protein, tyrosyl DNA phosphodiesterase 1, the colchicine binding site in tubulin, adenosine A2A receptor, … " - B-tubulin molecular weight

B-tubulin molecular weight

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WebPA5-19489 detects alpha Tubulin which has a predicted molecular weight of approximately 50 kDa. Target Information Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha … WebMolecular docking demonstrated an efficient binding between these ligands and the colchicine-binding site on the tubulin. CA-61 formed two hydrogen bond interactions with THR 179 (B) and THR 353 (B), whereas two hydrogen bonds with LYS 254 (B) and 1 with ASN 101 (A) were identified for CA-84.

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WebApr 11, 2024 · Nutrition during the developmental stages has long-term effects on adult physiology, disease and lifespan, and is termed nutritional programming. However, the underlying molecular mechanisms of nutritional programming are not yet well understood. In this study, we showed that developmental diets could regulate the lifespan of adult … WebSpecificity The immunogen used for this product shares 75% homology with TUB (Tubby protein homolog, Uniprot: P50607). In western blot, we observe a specific band at ~55kDa which is not seen in KO cell lines. An additional band below this band of interest is seen at ~50kDa in both the WT and KO cells which could correspond to the protein TUB.

WebMicrotubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. Alpha and beta tubulin dimers … WebAlpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter with a theoretical molecular weight of 50 kDa. Tyrosine ligase adds a …

WebDec 5, 2024 · LWJ-M30 also dramatically decreased the level of polymeric tubulin, while the disruption of microtubules caused the cell cycle to be arrested in the G2/M phase. ... DM1-HKD with correct molecular weight and high purity (>98%) was obtained, which was confirmed by ESI-MS and analytical HPLC (Fig. 1B and C).

WebOur previous study found that 2-phenyl-4-quinolone (2-PQ) derivatives are antimitotic agents, and we adopted the drug design concept of scaffold hopping to replace the 2-aromatic ring of 2-PQs with a 4-aromatic ring, representing 4-phenyl-2-quinolones (4-PQs). The 4-PQ compounds, whose structural backbones also mimic analogs of …

WebBeta-Tubulin, is usually used as loading control for Western Blot to normalize the levels of protein detected by confirming that protein loading is the same across the gel. OriGene … hacker school germanyWebThis antibody detects a single clean band at just above 40kD that corresponds to beta Actin. Please note this antibody is raised against the b-nonmuscle isoform and does not stain adult cardiac and skeletal muscles, except for traces due to contaminations of the sample with non-muscle cells, or if embryonic tissue is being used. hacker school cehWebIn oncology, class III β-tubulin has been investigated as both a prognostic biomarker and an indicator of resistance to taxanes and other compounds. The majority of reports implicate … hackers charactersWebApr 13, 2024 · Molecular weight standards are included at left, in units of kDa. Representative image is shown out of 5 biological replicates. ... α-tubulin; dark gray, β-tubulin). b Similar to a, ... hackers chineseWebAll lanes : Anti-alpha Tubulin antibody - Microtubule Marker (ab18251) at 0.5 µg/ml Lane 1 : HeLa (Human epithelial carcinoma cell line) whole cell lysate Lane 2 : HEK-293 (Human embryonic kidney cell line) whole cell lysate Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) whole cell lysate Lane 4 : Caco-2 (Human colonic carcinoma cell … hackers character namesWebAnti-Beta III Tubulin Antibody. Background Information. Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, … hackers chatgptWebSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. Protocol … hackers champstudy co kr